AptaIT offers bioinformatic services for the innovative analysis of NGS data from BCR or antibody sequencing experiments.
Deep sequencing of B-cell repertoires (B-cell receptor sequencing) offers the potential for a qualitative as well as quantitative understanding of the adaptive immune system in healthy and disease states or can be exploited for the enhanced identification of therapeutic and diagnostic antibodies. Recent advances in next-generation sequencing (NGS) have transformed the ability to examine antigen receptor repertoires at a single amino acid resolution. AptaIT offers analysis services for deep sequencing data of B-cell receptors of humans, mouse, rat, or rabbit. We will review the raw BCR (antibody) sequencing data, structure, parse it into a database, and therewith provide quick and intuitive access to analysis results in the form of tables and graphs. Both, investigation of a single snapshot of the immune system as well as comparison of multiple time points, e.g. for monitoring disease progression or drug response can be performed on a monoclonal IgG level. Comfortable functionalities enable to compare different individuals and the collection and identification of potential BCR-biomarkers.
The ultra-high resolution of deep sequencing of the B-cell receptor repertoires enables the comparison of the BCR status of all individuals/time points of all of your experiments. The systematic collection of BCR data in databases allows flexible comparison of current experiments with all others, which you performed in the past. Sequence analysis is possible for V-, CDR- or J-Regions of BCR heavy and light chains. Digital spectratyping enables quick access to relevant CDR3 regions. Furthermore, the identification of rare but relevant BCRs by intelligent clustering of receptor families or sequence tracking functions becomes possible. The annotation of V- and J-region is possible according to IMGT-standard. Learn more in our application note for BCR sequencing analysis.